Effect of various pathophysiological agents on the expression of TFPI activity by PASMC. Quiescent PASMC monolayers were treated for 24 hours with one of the following agents: PMA (10 ng/mL), serum (10% vol/vol), LPS (1 μg/mL), TNF- (10 ng/mL), thrombin (5 U/mL; 1.5 μg/mL), TGF-β (10 ng/mL), IL-1β (10 ng/mL), bFGF (10 ng/mL), and heparin (15 U/mL). In control, no agonist was added. The conditioned media, removed at the end of 24 hours of treatment, was assayed for TFPI activity. The data shown are the mean of three experiments performed in duplicate ± SD.