Surface phenotype of normal human blood Mo-derived DCs. DCs were cultured in RPMI-1640 medium plus 7.5% FCS in the presence of GM-CSF (500 U/mL), IL-4 (100 U/mL), and TNF- (50 U/mL) for 5 days. After culture, the cells were washed and then stained with various antibodies as described in Materials and Methods. The data are shown as histograms depicting the number of cells exhibiting various fluorescence intensities. The dotted lines represent staining with specific antibodies and the solid lines represent the isotype-matched control. Results are representative of 3 independent experiments.