RT-PCR of surgical tissue from the patient. RT-PCR using primers designed to amplify the lectin and epidermal growth-like domains was conducted on total cellular RNA isolated from surgical tissue from the patient that included normal tissue from the margins of the surgical amputation (lane 3) and ulcerated nonhealing inflamed tissue from two distinct sites (lanes 4 and 5). PCR with these primers of E-selectin cDNA (lane 2) yielded the expected product of 870 bp. An identical product was also identified in tissue samples from the abnormal, inflamed tissue (lanes 4 and 5) but was not readily detected in normal tissue at the surgical margin (lane 3).