Table 1.
Subcellular Distribution of Internalized OVAg and cHRP
| Type 1 | Type 2 | Type 3 | Type 4 | |
|---|---|---|---|---|
| WT/γY65F, Y76F OVAg | 9 ± 3 | 11 ± 4 | 21 ± 5 | 59 ± 13 |
| Δ315/γY65F, Y76F OVAg | 24 ± 2 | 18 ± 1 | 13 ± 1 | 45 ± 2 |
| WT/γY65F, Y76F cHRP | 11 ± 4 | 12 ± 4 | 21 ± 1 | 56 ± 10 |
| Δ315/γY65F, Y76F cHRP | 9 ± 1 | 15 ± 2 | 29 ± 3 | 47 ± 1 |
| WT/γ-chain OVAg | 5 ± 1 | 6 ± 2 | 58 ± 7 | 31 ± 7 |
| Δ315/γ-chain OVAg | 11 ± 6 | 12 ± 6 | 21 ± 2 | 56 ± 11 |
| Type 1 | Type 2 | Type 3 | Type 4 | |
|---|---|---|---|---|
| WT/γY65F, Y76F OVAg | 9 ± 3 | 11 ± 4 | 21 ± 5 | 59 ± 13 |
| Δ315/γY65F, Y76F OVAg | 24 ± 2 | 18 ± 1 | 13 ± 1 | 45 ± 2 |
| WT/γY65F, Y76F cHRP | 11 ± 4 | 12 ± 4 | 21 ± 1 | 56 ± 10 |
| Δ315/γY65F, Y76F cHRP | 9 ± 1 | 15 ± 2 | 29 ± 3 | 47 ± 1 |
| WT/γ-chain OVAg | 5 ± 1 | 6 ± 2 | 58 ± 7 | 31 ± 7 |
| Δ315/γ-chain OVAg | 11 ± 6 | 12 ± 6 | 21 ± 2 | 56 ± 11 |
Transfectants were incubated with OVAgold (OVAg) or cHRP as described in Materials and Methods. cHRP was visualized on ultrathin cryosections with an HRP antibody and 10-nm protein A gold particles. Cell profiles were randomly selected, and gold particles were assigned to a compartment when within 20 nm of a membrane. Percentages of gold particles ± SEM, based on three to four analyses of 20 cell profiles, present in each compartment are shown. Four types of compartments were distinguished, ie, incoming and recycling membranes (type 1), early endosomes (type 2), multivesicular late endosomes (type 3), and lysosomes (type 4).
