Cytological characteristics of 10-week and 40-week cultured cells grown by SCF. Cytochemical and immunologic stainings of the cultured cells grown by 10 ng/mL of SCF were performed on cytocentrifuged samples. Staining of 10-week cultured cells (A) and 40-week cultured cells (B) with May-Grünwald-Giemsa. Staining of 10-week cultured cells (C) and 40-week cultured cells (D) with an MoAb for tryptase. Staining of 10-week cultured cells (E) and 40-week cultured cells (F) with an MoAb for chymase. (Original magnification × 1,000.)