American Society of Hematology

Fig. 2.

$Fig. 2. Immunoblot analysis of murine BM neutrophils. Murine BM neutrophils were obtained from wild-type mice (WT), X-CGD mice (CGD), and X-CGD mice at various times posttransplantation with MSCV-m91–transduced BM (11, 12—18 months; 13—16 months; 26, 27—11 months). Extracts from WT neutrophils were loaded at 5, 2.5, and 1.25 μg; 5 μg of X-CGD neutrophil extracts were loaded. Expression of murine gp91phox (indicated by the arrow) was analyzed by immunoblotting with a rabbit polyclonal antiserum raised against the carboxy terminus of gp91phox. Additional immunoreactive bands are presumed to represent proteins that bind nonspecifically to the antiserum as they are also present in control X-CGD samples, are localized in the cytosol rather than membrane fraction (not shown), and are not detected with other gp91phox-specific antibodies (not shown).$

Immunoblot analysis of murine BM neutrophils. Murine BM neutrophils were obtained from wild-type mice (WT), X-CGD mice (CGD), and X-CGD mice at various times posttransplantation with MSCV-m91–transduced BM (11, 12—18 months; 13—16 months; 26, 27—11 months). Extracts from WT neutrophils were loaded at 5, 2.5, and 1.25 μg; 5 μg of X-CGD neutrophil extracts were loaded. Expression of murine gp91^phox (indicated by the arrow) was analyzed by immunoblotting with a rabbit polyclonal antiserum raised against the carboxy terminus of gp91^phox. Additional immunoreactive bands are presumed to represent proteins that bind nonspecifically to the antiserum as they are also present in control X-CGD samples, are localized in the cytosol rather than membrane fraction (not shown), and are not detected with other gp91^phox-specific antibodies (not shown).

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