Fig. 4.
Fig. 4. RP-HPLC separation of peptides from Lys C digests of trocarin subunits. (A) LC-MS profile of peptides from light chain digest on a Hypersil BDS C18 column. Flow rate: 50 μL/min. (B) HPLC profile of peptides from heavy chain digest on a Jupiter C18 column. Flow rate: 1 mL/min. Peptides were eluted with a linear gradient of buffer B (80% ACN in 0.1% TFA).

RP-HPLC separation of peptides from Lys C digests of trocarin subunits. (A) LC-MS profile of peptides from light chain digest on a Hypersil BDS C18 column. Flow rate: 50 μL/min. (B) HPLC profile of peptides from heavy chain digest on a Jupiter C18 column. Flow rate: 1 mL/min. Peptides were eluted with a linear gradient of buffer B (80% ACN in 0.1% TFA).

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