Protein phosphorylation upon platelet activation by MWReg30. Tyrosine-phosphorylated proteins from mouse platelets were separated by gel electrophoresis. (A) Plain platelet lysates without (lane 1) or after 5 minutes of pre-exposure of the platelets to MWReg30 (30 μg/mL) (lane 2) stained for phosphotyrosin after blotting. (B) Immunoprecipitates of gpIIb/IIIa from platelet lysates with MWReg30 without (lane 1 and 3) and after 5 minutes of pre-exposure of the platelets to MWReg30 (30 μL/mL) stained for protein detection with Coomassie (lanes 1 and 2) or for phosphotyrosin after blotting (lanes 3 and 4).