Desensitization for the MWReg30-induced hypothermia by LPS or TNF pretreatment. Hypothermia induced by IP injection of MWReg30 (100 μg) was determined in mice after pretreatment with (A) PBS (200 μL), (B) LPS (10 μg), or (C) rmTNF (600 ng) IP 24 hours before challenge. The mean values of the body temperatures of 3 mice per group at the indicated times after injection of the antibodies from 1 of 2 experiments are given ± SD.