Enhancing effect of CyPB on platelet adhesion. Washed platelets were incubated in the presence of increasing concentrations of CyPB in the absence (•) or presence of 500 nmol/L (○) or 5 μmol/L (□) of CsA and added to 96-well plates coated with collagen (1 μg/ well) for a 30-minute incubation at 37°C. After washing, adherent platelets were quantified using a BCA protein assay and expressed as percentages of initially added platelets (1 × 10⁷ platelets per well). Results are expressed as mean values ± SEM from quadruplicates and are representative from at least 3 separate experiments conducted with platelets from different donors.