Flow cytometric analysis of cell-surface markers on wild-type, Jak3−/+, and Jak3−/− mice. (A) Dot plots of peripheral blood leukocytes (PBLs) depicting FSC and SSC scatter, R1 gates live cells from dead cells, and the R6 gate denotes the normal lymphocyte population. Density plots of PBLs stained with antibodies against FcγRII/III (vertical axis) versus Mac-1, Gr-1, and F4/80 (horizontal axes). (B) Dot plots of PBLs from Jak3−/− mice gating on large, granular cells (gate R4) and large, less granular cells (gate R5). Density plots of Jak3−/− PBLs show FcγRII/III, Gr-1, and F4/80 staining patterns of R4 and R5 populations.