C/EBP slows 32D cl3 cell proliferation. 32D cl3 32D cl3, 32D-Puro, 32D-WT-ER-1, and 32D-WT-ER-2 cells in IL-3 were cultured in the absence or presence of estradiol (− Est. or + Est.). Viable cell numbers were enumerated daily with a hemocytometer and Trypan Blue Dye. Results shown are the mean of three determinations. Standard errors (SEs) were less than 15% of each mean.