C/EBP rapidly induces PU.1 protein in 32D cl3 and Ba/F3 cells. (A) Protein extracts were prepared from 32D cl3 and 32D-WT-ER-2 cells in IL-3 exposed to estradiol for 0 hours, 4 hours, 8 hours, 1 day, 2 days, 3 days, or 4 days. These extracts were subjected to Western blotting for PU.1. Fast Green staining of the blot is shown as a control for protein loading. (B) Protein extracts were prepared from Ba/F3(G) and Ba/F3(G)-WT-ER cells cultured in IL-3 and exposed to estradiol for 0 hours, 4 hours, 8 hours, 1 day, 2 days, 3 days, or 4 days. These extracts were also subjected to Western blotting for PU.1.