American Society of Hematology

Fig. 5.

$Fig. 5. (A) Intracellular bFGF expression pattern of PBMC from HCL patients. A total of 30 μL of cell lysates (2 × 106cells/mL) were separated by SDS-PAGE, blotted, and analyzed by Western blotting. PBMC from HCL expressed three bFGF isoforms (approximately 18, 23, and 25 kD), while no bFGF could be detected in HD. rhbFGF was used as control. (B) Release of a 23-kD bFGF isoform. CM from HD and HCL cultures was harvested after 48 hours, subjected to heparin chromatography, and bulk eluted fractions were separated by SDS-PAGE. Proteins were transferred onto nitrocellulose and analyzed by Western blotting. rhbFGF served as a positive control.$

(A) Intracellular bFGF expression pattern of PBMC from HCL patients. A total of 30 μL of cell lysates (2 × 10⁶cells/mL) were separated by SDS-PAGE, blotted, and analyzed by Western blotting. PBMC from HCL expressed three bFGF isoforms (approximately 18, 23, and 25 kD), while no bFGF could be detected in HD. rhbFGF was used as control. (B) Release of a 23-kD bFGF isoform. CM from HD and HCL cultures was harvested after 48 hours, subjected to heparin chromatography, and bulk eluted fractions were separated by SDS-PAGE. Proteins were transferred onto nitrocellulose and analyzed by Western blotting. rhbFGF served as a positive control.

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