Effect of 2-CdA on proliferation, bFGF release, and apoptosis of HC. (A) A total of 2 × 105 cells/well were cultured in medium or medium supplemented with bFGF (100 pg/mL) with or without 2-CdA (1 μg/mL). After 72 hours, cells were pulsed with 5 μCi/mL 3H-TdR and incorporation was measured by liquid scintillation counting. Mean values of triplicate cultures are shown. (B) A total of 2 × 106 PBMC from two patients with HCL were cultured in 6-well plates in the absence or presence of 2-CdA (1 μg/mL). After 48 hours, culture supernatants were screened by an ELISA specific for human bFGF. Results are expressed as mean values of triplicate cultures. (C) A total of 2 × 106 PBMC from a healthy donor and a patient with HCL were cultured in medium or medium containing 2-CdA (1 μg/mL, 0.1 μg/mL). Cells were harvested after 48 hours and assayed for FITC-labeled Annexin binding by FACS. Shown are results of a representative experiment.
