Fig. 1.
Fig. 1. Characterization of retroviral vectors. (A) Schematic diagram of HOXA5 and control vectors with FACS analysis of tNGFR expression (MC192-PE-GAM fluorescence) in corresponding PG13 vector-producing clones. (B) Ethidium bromide gel showing products of reverse PCR from RNA extracted from vector producing PG13 clones (lanes 3 through 10). The 193-bp HOXA5product is detected in lane 1 (a positive control using vector plasmid) and lanes 3 and 4, showing that HOXA5 expression is present in PG13 clones containing HOXA5 vectors and not in PG13 clones with control vectors. (+/−RT, reverse transcriptase added/not added). (C) Southern blot analyses of 293A cells transduced with vectors shown, after digestion of DNA with EcoR5, which cuts at points 3′ and 5′ to the HOXA5 cDNA, and probing with HOXA5 cDNA.

Characterization of retroviral vectors. (A) Schematic diagram of HOXA5 and control vectors with FACS analysis of tNGFR expression (MC192-PE-GAM fluorescence) in corresponding PG13 vector-producing clones. (B) Ethidium bromide gel showing products of reverse PCR from RNA extracted from vector producing PG13 clones (lanes 3 through 10). The 193-bp HOXA5product is detected in lane 1 (a positive control using vector plasmid) and lanes 3 and 4, showing that HOXA5 expression is present in PG13 clones containing HOXA5 vectors and not in PG13 clones with control vectors. (+/−RT, reverse transcriptase added/not added). (C) Southern blot analyses of 293A cells transduced with vectors shown, after digestion of DNA with EcoR5, which cuts at points 3′ and 5′ to the HOXA5 cDNA, and probing with HOXA5 cDNA.

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