Fig. 3.
The effect of the concentration of DFO or 311 on iron uptake from 59Fe-transferrin (0.75 μmol/L) by BE-2 and SK-N-MC cells over (A) 3 hours and (B) 24 hours of incubation at 37°C. After this incubation, the cells were washed four times with ice-cold BSS and incubated with pronase (1 mg/mL) at 30 minutes for 4°C.21 43 Results are means of duplicate determinations in a typical experiment of two experiments performed.