Enhancement of IL-12Rβ2 and IFN-γ transcript expression in HIV-infected PBMC by IL-12 and IFN-. PBMC from eight patients were cultured at 2 × 10⁶/mL for 72 hours with 2 μg/mL PHA ± 2 ng/mL IL-12 or 1,000 U/mL IFN-. (A) RNA was extracted and RT-PCR was performed for the detection of IL-12Rβ1, IL-12Rβ2, IFN-γ, and HPRT mRNA. Representative results from 4 of 8 donors are shown. (B) Densitometric values were assigned to bands by ImageQuaNT, normalized to HPRT values, and displayed as fold induction of message levels compared with PHA stimulation alone. Data points are from individual donors (n = 8) and the mean is represented by (+).