Binding of HA to macrophage cells. After 4 weeks, LTBMC nonadherent cells were washed out, adherent cells were treated for 30 minutes at 37°C with HA’ase (5 U/mL), and then fixed with 3% paraformaldehyde and incubated with FITC-labeled HA at 4°C for 30 minutes. Immunofluorescence-staining of a representative cell is shown. Magnification was 1,000-fold. Similar staining was observed without HA’ase treatment.