Fig. 9.
HA-induced activation of proline-directed protein kinases in BMDM. BMDM were stimulated with HA (100 μg/mL) for the indicated times, then lysed, and protein samples were analyzed by Western blotting for the presence of phosphorylated p38 kinase and phosphorylated Erk. The same membranes were stripped and stained with anti p38 or Erk antibodies, respectively. Where indicated, cells were preincubated for 15 minutes at 4°C with KM81 F(ab′)2 fragments (20 μg/mL) before HA stimulation.
