Fig. 4.
Effect of GM-CSF on fMLP-induced p21rac activation. (A) Neutrophils were isolated as described and stimulated with the indicated final concentrations of fMLP for 10 seconds. p21rac activation was determined as described in the legend to Fig 1. One percent of a total cell lysate was used for comparison with the amount of activated p21rac. (B) Neutrophils were pretreated for 20 minutes with 10−10 mol/L GM-CSF after stimulation with either 10−8 mol/L fMLP (top) or 10−10 mol/L fMLP (bottom) for the time indicated.
