American Society of Hematology

Fig. 3.

$Fig. 3. Effect of homocysteine on vWF multimerization. HUVEC cultured in the absence or presence of 5.0 mmol/L homocysteine for 1, 2, 4, 8, or 18 hours were denatured in lysis buffer, the lysates fractionated on 1.2% (wt/vol) agarose gels and transferred to nitrocellulose. vWF monomers, dimers, and multimers were detected by immunoblotting using anti-vWF antibodies.$

Effect of homocysteine on vWF multimerization. HUVEC cultured in the absence or presence of 5.0 mmol/L homocysteine for 1, 2, 4, 8, or 18 hours were denatured in lysis buffer, the lysates fractionated on 1.2% (wt/vol) agarose gels and transferred to nitrocellulose. vWF monomers, dimers, and multimers were detected by immunoblotting using anti-vWF antibodies.

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