Fig. 4.
![Fig. 4. Homocysteine increases the stable association between GRP78 and vWF. HUVEC cultured in the absence or presence of 0.2, 1.0, or 5.0 mmol/L homocysteine for 8 or 18 hours were metabolically labeled for 1 hour in the presence of [35S]methionine and [35S]cysteine. Radiolabeled proteins from cell lysates were immunoprecipitated with anti-vWF antibodies, separated on 7.5% SDS polyacrylamide gel under reducing conditions, and detected by fluorography. Immunoprecipitation of pro and mature vWF coprecipitated GRP78. kD, molecular mass markers.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/3/10.1182_blood.v94.3.959.415k20_959_967/5/blod41520004w.jpeg?Expires=1735833443&Signature=LbGcqoJwKO7nUoCukrZLD5SDMgrQ8AhAAOItyE85eu9a0PmL9Ldf2oTUQIt-ypQbjrHHMdFrXcb9C6KU-6SjvbSU-y9Kztzz4vNkAsUOmwZ2vxOQ99~dcNqrIby63uvaD9Za2xU0qrxImoeKC9kYeDdyqpsihvspjQ29Hln1Y3P1mLRka~H4bbsk-MReK0Em1AgnYKEmXzSgGphWnE1MidugM6A7VMeB8Qd7R8SLNeg6mlY4NV0qrncj4QOU9durlqNh4Yh1gmnhoHauGLQcjxNAHxcNlYEkMIKrRK78ltRSDa8rfSC0FyuuvRX7B-5UIVdWf1a3v75cSaA1vyKq7w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Homocysteine increases the stable association between GRP78 and vWF. HUVEC cultured in the absence or presence of 0.2, 1.0, or 5.0 mmol/L homocysteine for 8 or 18 hours were metabolically labeled for 1 hour in the presence of [35S]methionine and [35S]cysteine. Radiolabeled proteins from cell lysates were immunoprecipitated with anti-vWF antibodies, separated on 7.5% SDS polyacrylamide gel under reducing conditions, and detected by fluorography. Immunoprecipitation of pro and mature vWF coprecipitated GRP78. kD, molecular mass markers.
