Effect of EE treatment on hepatic LRP and mannose receptor mRNA expression in mice. (A) Total RNA was extracted from livers from vehicle- (lane 1) or EE-treated mice (lane 2) and analyzed by Northern blotting for LRP and mannose receptor (MR) mRNA levels. As a control for equal loading, the blots were probed with the cDNA for GAPDH mRNA. (B) The signals for LRP and MR mRNA were quantified by phosphoimager analysis and adjusted for the corresponding GAPDH mRNA signals. The results shown are the amounts of hepatic LRP and MR mRNA in EE-treated mice relative to those found in vehicle-treated mice. Data are expressed as the mean ± SD of 3 independent experiments consisting of at least 4 animals in each treatment group.