RT-PCR amplification products of the indicated genes obtained with bone marrow samples from 8 different patients with MGUS (no. 5), myeloma stage I (no. 12), or myeloma stage III (nos. 21 through 39). C+, positive control melanoma line (MZ2-MEL for MAGE-A3, GAGE-3/6, and β-ACTIN; LB373-MEL for LAGE-1 and PRAME). C−, negative control (no RNA present in the RT reaction). MW, molecular weight marker is SmartLadder (Eurogentec, Seraing, Belgium). Amplifications of MAGE-A3, GAGE-3/6, PRAME, and β-ACTIN transcripts give unique bands of 725, 244, 561, and 615 bp, respectively. Amplification of LAGE-1 transcript gives 2 bands that correspond to fully spliced (399 bp) and partially spliced (628 bp) mRNA, respectively, and a band of approximately 600 bp representing heteroduplexes formed during PCR between the products amplified from the partially and the fully spliced cDNAs.