Fig. 6.
Calcium mobilization induced by F peptide in FPRL1/293 cells and attenuation by fMLF. FPRL1/293 cells were loaded with Fura-2 and stimulated with different concentrations of F peptide (A), and the ratio of fluorescence at 340 and 380 nm was recorded and calculated. (B) shows sequential stimulation of FPRL1/293 cells with F peptide and fMLF or vice versa. F peptide (5 × 10−6mol/L) did not induce calcium flux in 293 cells transfected with CCR5 (C) or CXCR4 (D) and neither did F peptide (5 × 10−6mol/L) attenuate the calcium flux induced by RANTES (200 ng/mL) or SDF-1 (200 ng/mL) in these cells.
