Effect of PF-4–derived peptides on the direct association of ¹²⁵I-PF-4 with FGF-2 or VEGF. Wells of 96-well plates are coated with 15 ng FGF-2 (A, B) or 35 ng VEGF (C, D) and incubated with 10 ng ¹²⁵I-PF-4 with or without 2 μg unlabeled PF-4, 10 μg anti–PF-4 antibodies, or peptides in 50 μL incubation volume. Solid-phase binding experiments are performed as indicated Materials and Methods with fixed (10 μmol/L) or increasing peptide concentrations. Peptide 47-70 (○), 34-58 (□). Results are expressed as the mean ± SD of experiments done in triplicates.