Multiple tissue Northern blot analysis for Bik/Nbk expression. PolyA+ RNA (2 μg) were loaded per lane. Arrows indicate the position of the 1.1 kb Bik/Nbk transcript. (A) Multiple human tissue Northern blot analysis. Lane 1, heart; 2, brain; 3, placenta; 4, lung; 5, liver; 6, skeletal muscle; 7, kidney; 8, pancreas. (B) Lane 1, spleen; 2, thymus; 3, prostate; 4, testis; 5, ovary; 6, small intestine; 7, colon; 8, peripheral blood leukocytes. (C) Human cell lines Northern blot analysis. Lane 1, promyelocytic leukemia HL60; 2, cervix carcinoma HeLa S3; 3, erythroid leukemia K562; 4, T-cell leukemia MOLT4; 5, type III Burkitt lymphoma Raji; 6, colorectal adenocarcinoma SW480; 7, lung carcinoma A549; 8, melanoma G361.