Expression and induction of the GR in H9 cells. (A) Western blot analysis for GR- (95 kD, upper band) and GR-β (90 kD, lower band) expression. Lane 1, H9 3D8 cells; lane 2, H9 Bik/Nbk clone no. 16, lane 3, clone no. 10; lane 4, clone no. 2. (B) Western blot analysis for induction of GR expression by dexamethasone in H9 3D8 cells. Lane 1, medium control; lane 2, 24-hour culture in the presence of dexamethasone (10⁻⁶ mol/L); lane 3, 48 hours; lane 4, 72-hour induction. (C) Induction of GR expression by dexamethasone in H9 Bik/Nbk clone no. 16. Lane 1, medium control; lane 2, 24-hour culture in the presence of dexamethasone (10⁻⁶ mol/L); lane 3, 48 hours; lane 4, 72 hours.