Fig. 1.
(A) Nucleotide and deduced amino acid sequences of theBm-spn-2 cDNA. Inferred amino acid numbering is in boldface. The putative hydrophobic signal peptide (amino acids 1-20) is underlined and 2 potential N-glycosylation sites (amino acids 21 and 360) are circled. The serpin motif (amino acids 353-357) and serpin signature (amino acids 377-387) are boxed. The position of a potential polyadenylation signal (nucleotide 1398-1404) is in boldface. Primers used in PCRs are indicated with solid arrow. The nucleotide sequence has been deposited in GenBank with the accession no. AF009825. (B) Schematic of the gene structure of Bm-spn-2. The gene spans 2.7 kb and is split into 7 exons and 6 introns, as depicted. The nucleotide sequence has been deposited in GenBank with the accession no. AF009826.