Fig. 3.
Transcription of Bm-spn-2 at different development stages of B malayi. RNAs isolated from L3, Mf, adult males (lane M), and adult females (lane F) were processed to obtain first-strand cDNA. The cDNAs were then used as templates for PCR amplification of a 425-bp fragment from Bm-spn-2 or a 575-bp fragment from B malayi tph-1.