Recombinant and native Bm-SPN-2 protein. Western blot of lysates of BL21(DE3) cells containing the pET-29/Bm-spn-2plasmid after IPTG induction (lane 1) and extracts of Mf (lane 2), L3 (lane 3), adult males (lane 4), and adult females (lane 5) probed with mouse antirecombinant Bm-SPN-2. Recognition of a 52-kD band in lane 1 and of a 47.5-kD band in lane 2 is arrowed.