Fig. 4.
![Fig. 4. Effect of MCP-1 on intracellular [Ca2+]i in differentiating monocytes. Cells (5 × 106/mL) were incubated with Indo-1 probe (2 μg/mL final) at 37°C for 30 minutes, washed, and exposed in cuvette (1 × 106/mL) to recombinant MCP-1 (10 ng/mL) or RANTES (10 ng/mL) or FMLP (1 × 10−7 mol/L) in the presence of 1 mmol/L extracellular Ca2+. One representative experiment of 3 is shown in (A). (B) Average values obtained in 3 independent experiments with monocytes from different donors.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/3/10.1182_blood.v94.3.875.415k28_875_883/5/blod41528004x.jpeg?Expires=1735544564&Signature=Qrsd5XiLeXskZyjznpmfHU-0Nzy8f-Nmc6W-j1qaI7S~qVpsnD51fXEjOWWHMAmrlMiGGdX65ePiew0PwO6N4saQbucGwCjUc2aMBrWYlRil4VN2yzy7XJwvjM5EygI1YbhhTJ3-c4PG95-yFffu40atK-eu5UV5Dfsi6OKIzTB5QlxhblyF6tIizTLPXeYYRGCcIFImBuHAd-iiLE5w96xDICGMw8W7R4ujo7NpzwZUsL9ryZAiEqzCNir01HXF81BCa06rJwpqAUHKhXjWU2BUm81fhFv2LXmX8pOab5RNEX63e-t2RigELYUMBwfYPuxFxqos~9tQUA6q2Lf5pA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Effect of MCP-1 on intracellular [Ca2+]i in differentiating monocytes. Cells (5 × 106/mL) were incubated with Indo-1 probe (2 μg/mL final) at 37°C for 30 minutes, washed, and exposed in cuvette (1 × 106/mL) to recombinant MCP-1 (10 ng/mL) or RANTES (10 ng/mL) or FMLP (1 × 10−7 mol/L) in the presence of 1 mmol/L extracellular Ca2+. One representative experiment of 3 is shown in (A). (B) Average values obtained in 3 independent experiments with monocytes from different donors.
