Fig. 2.
p73 gene expression in pre B/B-ALL, T-ALL/LBL, and B-NHL clinical samples. Autoradiography of RT-PCR products (390 bp) of the p73 gene is shown in the top panels and GAPDH in the bottom panels. (A) Lane 1, HL60; lanes 2 through 5, peripheral blood mononuclear cells from 4 volunteers; lanes 6 through 14, pre B-ALL samples. (B) Lane 1, HL60; lanes 2 through 9, T-ALL/LBL samples. (C) Lane 1, HL60; lanes 2 through 9, B-NHL samples (lane 2, follicle center lymphoma, follicular, grade I; lane 3, diffuse large B-cell lymphoma; lane 4, lymphoplasmacytiod lymphoma; lane 5, small lymphocytic lymphoma; lane 6, small lymphocytic lymphoma; lane 7, mantle cell lymphoma; lane 8, follicle center lymphoma, follicular, grade II; lane 9, follicle center lymphoma, follicular, grade I). Numbers below the lanes represent the ratio ofp73 expression compared with expression in HL60 cells. The asterisk (*) indicates p73 expression was less than 1/100 of the level observed in HL60 cells.