Structural analysis of p73 in leukemia and lymphoma. (A) PCR-SSCP analysis of p73-cDNA from cell lines. Sequencing of RT-PCR products of representative samples showed that no mutations were present and the differences in migration of the bands were derived from the polymorphisms at codons 336 and 349. Arrow (a) indicates the band appearing when codons 336 and 349 nucleotides are GCC and CAT, and arrow (b) indicates the band appearing when codons 336 and 349 nucleotides are GCT and CAC, respectively. (B) Southern blot analysis of the p73 gene from p73 nonexpressing lymphoid cell lines. (B1) A 0.8-kb EcoRI fragment of pBS-M13-p73, which covers exons 2 through 6, was used as a probe. Lanes 1 and 7, peripheral blood mononuclear cells; lanes 2 and 8, Hela; lanes 3 and 9, TALL-1; lanes 4 and 10, RPMI8402; lanes 5 and 11, ALL-Sil; lanes 6 and 12, BALL-1. (B2) Probe CD, which covers exons 7-9, was used as a probe. Lane 1, Raji; lane 2, HL60; lane 3, HUT78; lane 4, U937; lane 5, Jurkat; lane 6, SKW3; lane 7, TALL-1; lane 8, CEM; lane 9, ALL-Sil; lane 10, RPMI8402; lane 11, KOPT-K1; lane 12, DS179; lane 13, NALM18; lane 14, BALL-1. Lanes 6, 7, 9, 10, 12, 13, and 14 are p73-negative cell lines as measured by RT-PCR.