American Society of Hematology

Fig. 8.

$Fig. 8. The multimeric composition of multimerin synthesized by AtT 20 cells. The left panel shows multimerin radioimmunoprecipitates from platelets and AtT 20 cells (fractions as in Figs 6B and 7) analyzed using nonreduced agarose/acrylamide gels. The right panel shows a Western blot of cell lysate from AtT 20 cells, analyzed on 3% to 8% SDS-PAGE after treatment with different concentrations of DTT (the multimerin subunits proM and proM*, native multimers, and intermediary forms generated by partial reduction are indicated). Similar to platelet multimerin,6 the multimerin stored and secreted from AtT 20 cells was composed of different sized multimers (left panel). Only small amounts of proM that had not yet multimerized were detected in the cell lysates (right panel).$

The multimeric composition of multimerin synthesized by AtT 20 cells. The left panel shows multimerin radioimmunoprecipitates from platelets and AtT 20 cells (fractions as in Figs 6B and 7) analyzed using nonreduced agarose/acrylamide gels. The right panel shows a Western blot of cell lysate from AtT 20 cells, analyzed on 3% to 8% SDS-PAGE after treatment with different concentrations of DTT (the multimerin subunits proM and proM*, native multimers, and intermediary forms generated by partial reduction are indicated). Similar to platelet multimerin,6 the multimerin stored and secreted from AtT 20 cells was composed of different sized multimers (left panel). Only small amounts of proM that had not yet multimerized were detected in the cell lysates (right panel).

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