Fig. 6.
![Fig. 6. MEK inhibition has no obvious effect on murine megakaryocyte morphology and acetylcholinesterase activity in whole marrow cultures. Murine bone marrow cells were harvested and split into 2 flasks. Both were grown in serum-free media with 35 ng/mL murine TPO for 3 days. One culture contained 50 μmol/L PD 98059 and the other contained an equal amount of DMSO. Cytospin preparations were then performed and stained with Giemsa ([A] DMSO; [B] PD 98059) or acetylcholinesterase activity (brown staining; [C] DMSO; [D] PD 98059).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/4/10.1182_blood.v94.4.1273/4/blod41604006by.jpeg?Expires=1735839712&Signature=eCoOx7cotDXlywsWWj~0ENC~NlibHi2aoPvA8UBLoHtvc2tZKGWWZJ4bdZXRubG5FG6F~Xp8t~N~XhBfUaC3isuZxxZ0~k-EqE95eMponAaX7KnYfj4MkkSD4IG7zm7-KJJJa-Iuopon~FCZ0yrx40kARgHMhcss7B7v~KC0sjDhhwzS4WTelM0K2NExL1~cpMWl0DLohntSrrgPvsxgjgPZYhf1tCOer03MQqFo5G3t~w4R6cy~KF08JGoVave~slgdGHqKw00tzcahQJjeNB9p83gDc9hDZ0drSU39oWEOAyMRvI8NMsocJJZ6n9hOeztY81Z6u2zn5ol-6EWjRg__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
MEK inhibition has no obvious effect on murine megakaryocyte morphology and acetylcholinesterase activity in whole marrow cultures. Murine bone marrow cells were harvested and split into 2 flasks. Both were grown in serum-free media with 35 ng/mL murine TPO for 3 days. One culture contained 50 μmol/L PD 98059 and the other contained an equal amount of DMSO. Cytospin preparations were then performed and stained with Giemsa ([A] DMSO; [B] PD 98059) or acetylcholinesterase activity (brown staining; [C] DMSO; [D] PD 98059).
