Western immunoblots of expressed s-Kell. The left panel shows Western immunoblots with a rabbit polyclonal antibody to human Kell protein. Proteins present in the media from sf9 cells expressing wild-type, Jsa Kell phenotype and E582G mutant s-Kell were separated by SDS-PAGE. Molecular size markers are shown. Lane 1 is a control from medium of sf9 cells that were not infected. Lane 2 is medium from cells expressing from wild-type s-Kell. Lane 3 has medium from cells expressing Jsa Kell phenotype and lane 4 has medium from those expressing E582G mutant. The right panel shows proteins isolated on nickel columns eluted with imidazole and analyzed as described above. Lane 1 is from cells expressing Jsa Kell phenotype, lane 2 is from cells expressing wild-type, and lane 3 is from cells expressing the E582G mutant. Lane 4 is a control from uninfected sf9 cells.
