Fig. 7.
Effect of anti-vβ3 integrin antibody on endothelial CD31-induced enhancement of eosinophil adhesion to IL-4–stimulated vascular endothelial cells. IL-4–stimulated HUVECs were preincubated with anti-CD31 MoAb or control mouse IgG (10 μg/mL each) for 10 minutes at 37°C. After washing HUVECs, the adhesion assay of51Cr-labeled eosinophils to HUVECs was performed in the presence or absence of anti-vβ3 integrin MoAb (10 μg/mL). Data are the means ± SD for 6 experiments. *Significantly different from the mean value of the control response (*P < .001). **Significantly different from the mean value of the control response of anti-CD31 MoAb-pretreated HUVECs (**P < .001).