Fig. 4.
![Fig. 4. Demonstration of C/EBPβ and GATA-1 binding to the MBP promoter by gel-shift assay. Double-stranded oligonucleotides (lower panel) containing MBP promoter sequence from bp −93 to −58 (probe A), with mutated GATA-consensus site (probe B), mutated C/EBP-consensus site (probe C), and mutated GATA- and C/EBP-consensus sites (probe D) were end-labeled with [γ-32P] ATP and incubated with 1 μg double-stranded poly (dI-dC) in the presence of 1 μg purified maltose-binding protein (MBP)-C/EBPβ fusion protein (lanes 2 and 4 through 7) and 8 μg nuclear proteins from COS7 cells that were transiently transfected with a GATA-1 expression vector (lanes 3 through 7).](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/94/4/10.1182_blood.v94.4.1429/4/blod41613004w.jpeg?Expires=1735590727&Signature=Vs1Idg8LBYOCQAvJe1iVgjwFe2uCA2yQav4EJxv0dKXZhPkOTgP5KYFnK9-z2PvKqzSEol7D2aRqoUcYI32-rFNzYbfGRtPuBwD7DqqloyUdNQzTGwGN2L1aGuAg7Y2VpDACawfPFZRAIjxgTkxACWt7naCJef1hTaaWNVqmcpmT8E4ZbN4FwAAmkaE4UiBds840PpW8J6R1lh8T90ng5OWNF0zKERZwGleD3vzc7gjZm-jD9SdpgYezlivIQeLWBWYGBKxOEgBHuwTe2-eSIK~iT5GEq~7SfQ5lpqfGLlCTwSLz8jGBxrVBLNSQ2SkfRRPxf7FUVhHMxVnOIzUAVA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Demonstration of C/EBPβ and GATA-1 binding to the MBP promoter by gel-shift assay. Double-stranded oligonucleotides (lower panel) containing MBP promoter sequence from bp −93 to −58 (probe A), with mutated GATA-consensus site (probe B), mutated C/EBP-consensus site (probe C), and mutated GATA- and C/EBP-consensus sites (probe D) were end-labeled with [γ-32P] ATP and incubated with 1 μg double-stranded poly (dI-dC) in the presence of 1 μg purified maltose-binding protein (MBP)-C/EBPβ fusion protein (lanes 2 and 4 through 7) and 8 μg nuclear proteins from COS7 cells that were transiently transfected with a GATA-1 expression vector (lanes 3 through 7).
