Fig. 3.
Fig. 3. Bcl-xL blocks apoptosis and DEVDase activity in Jurkat cells. (A) Expression of Bcl-xL in transfected Jurkat cells. Cellular proteins from Jurkat stably transfected with empty vector (neo) or Bcl-xL expression vector were separated by SDS-PAGE and immunoblotted using anti–Bcl-x antibody. (B) Jurkat cells transfected with vector control or Bcl-xLexpression vector were treated without or with 10 μg/mL ELL-12 for 5 hours. Apoptosis was assessed by Hoechst staining. Results are the means ± SD of at least 3 independent experiments. (C) Activation of DEVD-specific caspases was measured by the cleavage of the fluorogenic substrate Ac-DEVD-AMC. Results are the means ± SD of at least 3 independent experiments.

Bcl-xL blocks apoptosis and DEVDase activity in Jurkat cells. (A) Expression of Bcl-xL in transfected Jurkat cells. Cellular proteins from Jurkat stably transfected with empty vector (neo) or Bcl-xL expression vector were separated by SDS-PAGE and immunoblotted using anti–Bcl-x antibody. (B) Jurkat cells transfected with vector control or Bcl-xLexpression vector were treated without or with 10 μg/mL ELL-12 for 5 hours. Apoptosis was assessed by Hoechst staining. Results are the means ± SD of at least 3 independent experiments. (C) Activation of DEVD-specific caspases was measured by the cleavage of the fluorogenic substrate Ac-DEVD-AMC. Results are the means ± SD of at least 3 independent experiments.

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