American Society of Hematology

Fig. 3.

$Fig. 3. Analysis of arachidonate release by TLC. Day-1 CD34+ cells labeled with 3H-arachidonic acid were incubated with either 5 μmol/L MAFP or DMSO diluent before priming for 10 minutes with 100 ng/mL SCF, followed by activation with 1 μmol/L A23187 for 20 minutes. The lipids were extracted, spotted onto the origin (0) of the TLC plate, and separated as described in Materials and Methods. The distribution of radioactivity in 1-cm fractions scraped from the plate is shown. The data shown are from a single experiment representative of 3 that were performed.$

Analysis of arachidonate release by TLC. Day-1 CD34⁺ cells labeled with ³H-arachidonic acid were incubated with either 5 μmol/L MAFP or DMSO diluent before priming for 10 minutes with 100 ng/mL SCF, followed by activation with 1 μmol/L A23187 for 20 minutes. The lipids were extracted, spotted onto the origin (0) of the TLC plate, and separated as described in Materials and Methods. The distribution of radioactivity in 1-cm fractions scraped from the plate is shown. The data shown are from a single experiment representative of 3 that were performed.

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