Fig. 5.
TUNEL assay and cell-cycle analysis to determine the presence of DNA fragmentation. Daudi and Raji cells were stimulated with 50 μmol/L t-BHP for 6 hours at 37°C. Apoptosis-permissive Daudi cells, but not apoptosis-resistant Raji cells, were nick-end labeled by TdT. Viability was determined by staining the unfixed cells with PI.