(A) Agarose gel of products of the clone-specific PCR using primer BE-1. Lane 1, skin sample DNA of BE; 2, blood sample DNA of BE; 3-5, skin sample DNA from CTCL patients (tester DNA); 6-8, blood sample DNA from CTCL patients (tester DNA). A specific PCR product (indicated by the arrow) is observed exclusively in lane 2. The additional band in lanes 2 and 5 represents a larger PCR product that, according to the size, is referred to as an unspecific amplificate. (B) Agarose gel of the sensitivity assay using primer JU-5 and 10-fold dilution of JM cell line DNA in PBMC from a healthy donor. A specific PCR product (indicated by the arrow) is found up to the dilution of 10 JM cells in 10⁶ PBMC (10^-5) in lane 6. (C) Results of the analysis of the fractionated clones (1-14, established from patient HO; 15-20, from BE): fraction A was sequenced directly, fraction B was amplified by clone-specific PCR using primer HO-2 and separated on an agarose gel. Nonconcordance between PCR and sequencing is observed in lane 1 and 19. M, marker (Hinc II digest of phi X 174); PCR, PCR result; SEQ, result of sequencing; ⁺, PCR product of expected size/sequence identical to the second allel of patient HO; ⁻, no PCR product of expected size/sequence not identical to the second allele of patient HO.