American Society of Hematology

Fig. 3.

$Fig. 3. Effect of anti-II MoAb on thrombin production in a tubular flow reactor coated with phospholipid vesicles. Glass capillaries were coated with phospholipid vesicles and incubated with 0.1 μmol/L prothrombin, 0.1 nmol/L factor Va, and 0.4 μmol/L of control or anti-II MoAb for 1 hour. The capillaries were perfused with 20 pmol/L factor Xa at a flow rate of 30 μL/min. Two-minute fractions were collected and the thrombin concentration was determined by the rate of hydrolysis of N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide.$

Effect of anti-II MoAb on thrombin production in a tubular flow reactor coated with phospholipid vesicles. Glass capillaries were coated with phospholipid vesicles and incubated with 0.1 μmol/L prothrombin, 0.1 nmol/L factor Va, and 0.4 μmol/L of control or anti-II MoAb for 1 hour. The capillaries were perfused with 20 pmol/L factor Xa at a flow rate of 30 μL/min. Two-minute fractions were collected and the thrombin concentration was determined by the rate of hydrolysis of N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide.

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