American Society of Hematology

Fig. 4.

$Fig. 4. Effect of LA IgGs on thrombin production in the phospholipid-coated flow reactor. Glass capillaries were coated with phospholipid vesicles and incubated with 0.1 μmol/L prothrombin, 0.1 nmol/L factor Va, and control or LA IgG for 1 hour. The LA IgG and corresponding control IgG concentrations were 5 μmol/L for LA4 and LA7; 10 μmol/L for LA2, LA5, and LA6; 20 μmol/L for LA1; and 50 μmol/L for LA3. The capillaries were perfused with 20 pmol/L factor Xa at a flow rate of 30 μL/min. Two-minute fractions were collected and the thrombin concentration was determined by the rate of hydrolysis of N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide.$

Effect of LA IgGs on thrombin production in the phospholipid-coated flow reactor. Glass capillaries were coated with phospholipid vesicles and incubated with 0.1 μmol/L prothrombin, 0.1 nmol/L factor Va, and control or LA IgG for 1 hour. The LA IgG and corresponding control IgG concentrations were 5 μmol/L for LA4 and LA7; 10 μmol/L for LA2, LA5, and LA6; 20 μmol/L for LA1; and 50 μmol/L for LA3. The capillaries were perfused with 20 pmol/L factor Xa at a flow rate of 30 μL/min. Two-minute fractions were collected and the thrombin concentration was determined by the rate of hydrolysis of N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide.

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