American Society of Hematology

Fig. 7.

$Fig. 7. Effect of an anti-II MoAb on thrombin production in flow. A glass capillary was coated with phospholipid vesicles and incubated with 0.1 μmol/L prothrombin and 0.4 μmol/L control or anti-II MoAb and was then perfused with 0.1 μmol/L prothrombin, 0.4 μmol/L control or anti-II MoAb, 2 nmol/L factor Va, and 1 nmol/L Xa at a flow rate of 30 μL/min (collecting 15-second fractions every 30 seconds). Thrombin concentration was determined by the rate of hydrolysis of N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide.$

Effect of an anti-II MoAb on thrombin production in flow. A glass capillary was coated with phospholipid vesicles and incubated with 0.1 μmol/L prothrombin and 0.4 μmol/L control or anti-II MoAb and was then perfused with 0.1 μmol/L prothrombin, 0.4 μmol/L control or anti-II MoAb, 2 nmol/L factor Va, and 1 nmol/L Xa at a flow rate of 30 μL/min (collecting 15-second fractions every 30 seconds). Thrombin concentration was determined by the rate of hydrolysis of N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide.

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