American Society of Hematology

Fig. 8.

$Fig. 8. Effect of LA IgGs on thrombin production in flow. Glass capillaries were coated with phospholipid vesicles and incubated with 0.1 μmol/L prothrombin and control or LA IgG and were then perfused with 0.1 μmol/L prothrombin, control, or LA IgG; 2 nmol/L factor Va; and 1 nmol/L Xa at a flow rate of 30 μL/min (collecting 15-second fractions every 30 or 60 seconds). Thrombin concentration was determined by the rate of hydrolysis of N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide. The concentration of LA IgG used in the experiment was that which resulted in maximal prothrombin binding (see Fig 5): 5 μmol/L for LA4 and LA7; 10 μmol/L for LA2, LA5, and LA6; 20 μmol/L for LA1; and 50 μmol/L for LA3.$

Effect of LA IgGs on thrombin production in flow. Glass capillaries were coated with phospholipid vesicles and incubated with 0.1 μmol/L prothrombin and control or LA IgG and were then perfused with 0.1 μmol/L prothrombin, control, or LA IgG; 2 nmol/L factor Va; and 1 nmol/L Xa at a flow rate of 30 μL/min (collecting 15-second fractions every 30 or 60 seconds). Thrombin concentration was determined by the rate of hydrolysis of N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide. The concentration of LA IgG used in the experiment was that which resulted in maximal prothrombin binding (see Fig 5): 5 μmol/L for LA4 and LA7; 10 μmol/L for LA2, LA5, and LA6; 20 μmol/L for LA1; and 50 μmol/L for LA3.

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