American Society of Hematology

Fig. 10.

$Fig. 10. Effect of LA IgGs in plasma on thrombin production in the phospholipid-coated flow reactor. (A) Phospholipid-coated capillaries were incubated with 50% normal plasma containing 10 μmol/L D-Phe-Pro-Arg-CH2Cl, 10 mmol/L CaCl2, and 25 μmol/L control or LA IgG for 1 hour. The capillaries were washed with 2 capillary volumes of HEPES buffer and were then perfused with 1 nmol/L factor Xa and 2 nmol/L factor Va at a flow rate of 30 μL/min. One-minute fractions were collected and the thrombin concentration was determined by the rate of hydrolysis of N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide. Results for LA1, LA5, and LA7 IgG are shown. (B) Total thrombin production was calculated for each LA IgG from the area under the curve of profiles of the type shown in (A).$

Effect of LA IgGs in plasma on thrombin production in the phospholipid-coated flow reactor. (A) Phospholipid-coated capillaries were incubated with 50% normal plasma containing 10 μmol/L D-Phe-Pro-Arg-CH₂Cl, 10 mmol/L CaCl₂, and 25 μmol/L control or LA IgG for 1 hour. The capillaries were washed with 2 capillary volumes of HEPES buffer and were then perfused with 1 nmol/L factor Xa and 2 nmol/L factor Va at a flow rate of 30 μL/min. One-minute fractions were collected and the thrombin concentration was determined by the rate of hydrolysis of N-p-Tosyl-Gly-Pro-Arg-p-nitroanilide. Results for LA1, LA5, and LA7 IgG are shown. (B) Total thrombin production was calculated for each LA IgG from the area under the curve of profiles of the type shown in (A).

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