ROS production by adhered human monocyte-derived macrophages after 1, 2, 4, and 24 hours in serum-free culture in the presence and absence of factor VIIa. (A) PMA-triggered ROS production. (B) Spontaneous ROS production, measured without PMA triggering. (•) Values for cells cultured in the presence of LPS; (○) values for cells cultured in the absence of LPS. Continuous lines show values in the presence of factor VIIa (2.5 μg/mL); dotted lines show values in the absence of factor VIIa. ROS production is expressed as mfu.